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Dual-component structural plasticity mediated by αCaMKII autophosphorylation on basal dendrites of cortical layer 2/3 neurones

Seaton, Gillian, Hodges, Gladys, de Haan, Annelies, Grewal, Aneesha, Pandey, Anurag, Kasai, Haruo and Fox, Kevin 2020. Dual-component structural plasticity mediated by αCaMKII autophosphorylation on basal dendrites of cortical layer 2/3 neurones. Journal of Neuroscience 40 (11) , pp. 2228-2245. 10.1523/JNEUROSCI.2297-19.2020
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Abstract

Sensory cortex exhibits receptive field plasticity throughout life in response to changes in sensory experience and offers the experimental possibility of aligning functional changes in receptive field properties with underpinning structural changes in synapses. We looked at the effects on structural plasticity of two different patterns of whisker deprivation in male and female mice: chessboard deprivation, which causes functional plasticity; and all deprived, which does not. Using 2-photon microscopy and chronic imaging through a cranial window over the barrel cortex, we found that layer 2/3 neurones exhibit robust structural plasticity, but only in response to whisker deprivation patterns that cause functional plasticity. Chessboard pattern deprivation caused dual-component plasticity in layer 2/3 by (1) increasing production of new spines that subsequently persisted for weeks and (2) enlarging spine head sizes in the preexisting stable spine population. Structural plasticity occurred on basal dendrites, but not apical dendrites. Both components of plasticity were absent in αCaMKII-T286A mutants that lack LTP and experience-dependent potentiation in barrel cortex, implying that αCaMKII autophosphorylation is not only important for stabilization and enlargement of spines, but also for new spine production. These studies therefore reveal the relationship between spared whisker potentiation in layer 2/3 neurones and the form and mechanisms of structural plasticity processes that underlie them.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Society for Neuroscience
ISSN: 0270-6474
Date of First Compliant Deposit: 7 January 2020
Date of Acceptance: 3 January 2020
Last Modified: 01 Jun 2020 16:00
URI: http://orca-mwe.cf.ac.uk/id/eprint/128280

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