Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils

Hallett, Maurice B., Roberts, Rhiannon E. and Dewitt, Sharon 2019. Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils. Neutrophil, Vol. 2087. Methods in Molecular Biology, New York: Humana Press, pp. 191-205. (10.1007/978-1-0716-0154-9_14)

Full text not available from this repository.

Abstract

The measurement and manipulation of cytosolic free Ca2+ of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca2+ signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils, and are discussed here, especially the use of fluorescent indicators of Ca2+ and photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Dentistry
Medicine
Publisher: Humana Press
ISBN: 9781071601532
ISSN: 1064-3745
Last Modified: 29 Feb 2020 16:47
URI: http://orca-mwe.cf.ac.uk/id/eprint/128192

Actions (repository staff only)

Edit Item Edit Item