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Nitroimidazole and oxygen derived radicals detected by electron spin resonance in hydrogenosomal and cytosolic fractions from Trichomonas vaginalis

Yarlett, Nigel, Rowlands, Christopher C, Evans, Jeffrey C, Yarlett, Nuriza C and Lloyd, David 1987. Nitroimidazole and oxygen derived radicals detected by electron spin resonance in hydrogenosomal and cytosolic fractions from Trichomonas vaginalis. Molecular and Biochemical Parasitology 24 (3) , 255--261. 10.1016/0166-6851(87)90157-5

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Abstract

Hydrogenosome-enriched from Trichomonas vaginalis reduce a number of nitroimidazole derivatives to their respective electron spin resonance-detectable nitro-anion radicals. In the presence of oxygen and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) a superoxide spin trapped adduct of DMPO was formed; the rate-determining step was the prior information of the nitro-anion radical. Oxygen-derived radicals were detected with cytosolic fractions from a metronidazole-resistant isolate (CDC-85) when incubated with NADH or NADPH as respiratory substrate. The requirement for superoxide dismutase and catalase to completely abolish formation of these signals suggests contributions from both superoxide and peroxide. No oxygen-derived radicals were observed with cytosolic fractions from a metronidazole-susceptible strain (C1-NIH)

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0166-6851
Date of Acceptance: 23 March 1987
Last Modified: 20 Feb 2020 14:15
URI: http://orca-mwe.cf.ac.uk/id/eprint/128061

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