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Adaptation of terminal respiration pathways of Tetrahymena pyriformis ST to growth with added iron or carbonyl cyanide m-chlorophenylhydrazone

Unitt, M.D., Scott, Robert and Lloyd, D 1983. Adaptation of terminal respiration pathways of Tetrahymena pyriformis ST to growth with added iron or carbonyl cyanide m-chlorophenylhydrazone. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 74 (3) , 567--571. 10.1016/0305-0491(83)90230-4

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Abstract

1. Tetrahymena pyriformis grown in medium which contained 0.6 μg/ml iron possesses at least three pathways of terminal respiration which are inhibited by cyanide, azide and salicythydroxamic acid respectively.2.2. Mid-exponential phase cultures grown in the presence of 5.6 μg/ml iron and glucose showed the same respiratory properties in terms of respiration rate and inhibitor sensitivity as cultures grown in the absence of added iron and glucose.3.3. The respiration of mid-exponential phase cultures adapted to growth in the presence of 2.5 μm-carbonyl cyanide m-chlorophenylhydrazone was incompletely inhibited by a combination of cyanide, azide and salicylhydroxamic acid.4.4. The residual respiration accounted for between 25 and 35% of the total, indicating synthesis of a novel terminal oxidase or gross modification of existing terminal respiratory pathways in the presence of the uncoupler of energy conservation.5.5. The sole functional cytochrome oxidase present in organisms grown in the absence or presence of added iron and glucose or after adaptation to uncoupler was cytochrome a620; no other CO-reacting haemoprotein was detected in photochemical action spectra.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
ISSN: 1096-4959
Last Modified: 21 Feb 2020 10:45
URI: http://orca-mwe.cf.ac.uk/id/eprint/128042

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