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Oxygen induces fatty acid (n-6)-desaturation independently of temperature in Acanthamoeba castellanii.

Thomas, K, Rutter, A, Suller, M, Harwood, J and Lloyd, D 1998. Oxygen induces fatty acid (n-6)-desaturation independently of temperature in Acanthamoeba castellanii. FEBS Letters 425 (1) , 171--174. 10.1016/s0014-5793(98)00218-x

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Abstract

Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperature in batch cultures grown to O2-limitation. Raising the oxygen concentration from below the lower limit of detection (< 0.1 microM) to approximately air-saturation (230 microM), whilst maintaining the growth temperature constant (30 degrees C), increased lipid unsaturation and elevated n-6-desaturase activity 2.3-fold. Addition of the protein synthesis inhibitor, anisomycin, showed that increased desaturase activity was due to new protein synthesis rather than activation of pre-existing enzyme. These observations are important for future studies of the mechanism of temperature adaptation in poikilotherms.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0014-5793
Last Modified: 21 Feb 2020 13:30
URI: http://orca-mwe.cf.ac.uk/id/eprint/128037

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