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Acanthamoeba castellanii: growth, encystment, excystment and biocide susceptibility.

Khunkitti, W, Lloyd, D, Furr, J R and Russell, A D 1998. Acanthamoeba castellanii: growth, encystment, excystment and biocide susceptibility. Journal of Infection 36 (1) , 43--48. 10.1016/s0163-4453(98)93054-7

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Abstract

Stages in the encystment and excystment processes of Acanthamoeba castellanii have been studied. The kinetics of encystment involved measurements of the three phases (pre-encystment, cyst initiation and cyst wall synthesis). Excystment, starting from a mature cyst, involved pre-emergence, penetration outwards of the cyst wall and free trophozoite. The sensitivity to biocides of trophozoites in the exponential growth phase, pre-encystment trophozoites, mature cysts and pre-excystment cysts has been investigated. Some differences in relative sensitivity to a bisbiguanide (chlorhexidine) and a polymeric biguanide (polyhexamethylene biguanide) were observed, but mature cysts were always the most resistant cellular form.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: WB Saunders
ISSN: 0163-4453
Date of Acceptance: 24 January 1997
Last Modified: 21 Feb 2020 12:00
URI: http://orca-mwe.cf.ac.uk/id/eprint/127897

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