Human cytomegalovirus genomes sequenced directly from clinical material: Variation, multiple-strain infection, recombination, and gene loss

Suárez, Nicolás M, Wilkie, Gavin S, Hage, Elias, Camiolo, Salvatore, Holton, Marylouisa, Hughes, Joseph, Maabar, Maha, Vattipally, Sreenu B, Dhingra, Akshay, Gompels, Ursula A, Wilkinson, Gavin W G, Baldanti, Fausto, Furione, Milena, Lilleri, Daniele, Arossa, Alessia, Ganzenmueller, Tina, Gerna, Giuseppe, Hubacek, Petr, Schulz, Thomas F, Wolf, Dana, Zavattoni, Maurizio and Davison, Andrew J 2019. Human cytomegalovirus genomes sequenced directly from clinical material: Variation, multiple-strain infection, recombination, and gene loss. Journal of Infectious Diseases 220 (5) , pp. 781-791. 10.1093/infdis/jiz208

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Abstract

The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	University of Chicago Press / Oxford University Press (OUP): Policy A1 - Oxford Open Option C
ISSN:	0022-1899
Date of First Compliant Deposit:	3 October 2019
Date of Acceptance:	24 April 2019
Last Modified:	05 May 2023 08:54
URI:	https://orca.cardiff.ac.uk/id/eprint/125852

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