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High endothelial venules are associated with microsatellite instability, hereditary background and immune evasion in colorectal cancer

Pfuderer, Pauline L., Ballhausen, A, Seidler, F, Stark, H, Grabe, N, Frayling, I, Ager, A, Doeberitz, M, Kloor, M and Ahadova, A 2019. High endothelial venules are associated with microsatellite instability, hereditary background and immune evasion in colorectal cancer. British Journal of Cancer 121 , pp. 395-404. 10.1038/s41416-019-0514-6
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Abstract

Background: Microsatellite-unstable (MSI) tumours show a high load of mutational neoantigens, as a consequence of DNA mismatch repair deficiency. Consequently, MSI tumours commonly present with dense immune infiltration and develop immune evasion mechanisms. Whether improved lymphocyte recruitment contributes to the pronounced immune infiltration in MSI tumours is unknown. We analysed the density of high endothelial venules (HEV) and postcapillary blood vessels specialised for lymphocyte trafficking, in MSI colorectal cancers (CRC). Methods: HEV density was determined by immunohistochemical staining of FFPE tissue sections from MSI (n = 48) and microsatellite-stable (MSS, n = 35) CRCs. Associations with clinical and pathological variables were analysed. Results: We found elevated HEV densities in MSI compared with MSS CRCs (median 0.049 vs 0.000 counts/mm2, respectively, p = 0.0002), with the highest densities in Lynch syndrome MSI CRCs. Dramatically elevated HEV densities were observed in B2M-mutant Lynch syndrome CRCs, pointing towards a link between lymphocyte recruitment and immune evasion (median 0.485 vs 0.0885 counts/mm2 in B2M-wild-type tumours, p = 0.0237). Conclusions: Our findings for the first time indicate a significant contribution of lymphocyte trafficking in immune responses against MSI CRC, particularly in the context of Lynch syndrome. High HEV densities in B2M-mutant tumours underline the significance of immunoediting during tumour evolution.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Cancer Research UK
ISSN: 0007-0920
Date of First Compliant Deposit: 17 July 2019
Date of Acceptance: 3 June 2019
Last Modified: 18 Oct 2019 10:24
URI: http://orca-mwe.cf.ac.uk/id/eprint/124275

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