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Analysis of isothermal titration calorimetry data for complex interactions using I2CITC

Saeed, Ibrahim Q. and Buurma, Niklaas J. 2019. Analysis of isothermal titration calorimetry data for complex interactions using I2CITC. In: Ennifar, Eric ed. Microcalorimetry of Biological Molecules, Vol. 1964. Methods in Molecular Biology, Humana Press, pp. 169-183. (10.1007/978-1-4939-9179-2_13)

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Abstract

I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Chemistry
Publisher: Humana Press
ISBN: 9781493991785
ISSN: 1064-3745
Last Modified: 28 Jun 2019 02:04
URI: http://orca-mwe.cf.ac.uk/id/eprint/122094

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