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Profiling and simultaneous quantitative determination of anthocyanins in wild myrtus communisL. berries from different geographical areas in sardinia and their comparative evaluation

Maldini, Mariateresa, Chessa, Mario, Petretto, Giacomo L., Montoro, Paola, Rourke, Jonathan P., Foddai, Marzia, Nicoletti, Marcello and Pintore, Giorgio 2016. Profiling and simultaneous quantitative determination of anthocyanins in wild myrtus communisL. berries from different geographical areas in sardinia and their comparative evaluation. Phytochemical Analysis 27 (5) , pp. 249-256. 10.1002/pca.2623

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Abstract

Introduction Myrtus communis L. (Myrtaceae) is a self‐seeded shrub, widespread in Sardinia, with anti‐inflammatory, antiseptic, antimicrobial, hypoglycemic and balsamic properties. Its berries, employed for the production of sweet myrtle liqueur, are characterised by a high content of bioactive polyphenols, mainly anthocyanins. Anthocyanin composition is quite specific for vegetables/fruits and can be used as a fingerprint to determine the authenticity, geographical origin and quality of raw materials, products and extracts. Objective To rapidly analyse and determine anthocyanins in 17 samples of Myrtus communis berries by developing a platform based on the integration of UHPLC–MS/MS quantitative data and multivariate analysis with the aim of extracting the most information possible from the data. Methodology UHPLC‐ESI‐MS/MS methods, working in positive ion mode, were performed for the detection and determination of target compounds in multiple reaction monitoring (MRM) mode. Optimal chromatographic conditions were achieved using an XSelect HSS T3 column and a gradient elution with 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Principal component analysis (PCA) was applied to the quantitative data to correlate and discriminate 17 geographical collections of Myrtus communis. Results The developed quantitative method was reliable, sensitive and specific and was successfully applied to the quantification of 17 anthocyanins. Peonidin‐3‐O‐glucoside was the most abundant compound in all the extracts investigated. Conclusion The developed methodology allows the identification of quali‐quantitative differences among M. communis samples and thus defines the quality and value of this raw material for marketed products. Moreover, the reported data have an immediate commercial value due to the current interest in developing antioxidant nutraceuticals from Mediterranean plants, including Sardinian Myrtus communis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Publisher: Wiley
ISSN: 0958-0344
Date of First Compliant Deposit: 17 January 2019
Date of Acceptance: 4 April 2016
Last Modified: 29 Jun 2019 12:54
URI: http://orca-mwe.cf.ac.uk/id/eprint/118466

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