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Immunocytochemical and pharmacological evidence for GABAergic spiking local interneurons in the locust

Watson, Alan and Burrows, M 1987. Immunocytochemical and pharmacological evidence for GABAergic spiking local interneurons in the locust. Journal of Neuroscience 7 (6) , pp. 1741-1751. 10.1523/JNEUROSCI.07-06-01741.1987

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Abstract

In the locust thoracic nervous system, spiking local interneurons within a ventral midline population are stained by a polyclonal antibody raised against GABA. Their cell bodies, their primary neurites in ventral commissure II, and their prominent neurites in the perpendicular tract linking ventral and dorsal fields of fine branches are all stained. Individual interneurons in this population were labeled with Lucifer yellow after their receptive fields had been determined physiologically. Alternate sections were stained with the antibody. Some, but not all, of the spiking local interneurons labeled with Lucifer yellow are also stained with the antibody. Apart from having somata that lie more posteriorly within the midline population, the antibody-stained interneurons cannot be distinguished on morphological or physiological grounds from those that are unstained. The way in which this cytochemical heterogeneity within an otherwise homogenous population might arise during development is discussed. A second group of spiking local interneurons with similar sensory input to that of the ventral midline population, but with cell bodies in the anterior lateral region of the ganglion and primary neurites in ventral commissure I, are not stained. Interneurons in the midline group receive direct inputs from sensory neurons and some directly inhibit particular leg motor neurons that mediate interjoint and tactile reflexes. Picrotoxin reversibly blocks the inhibition of the motor neurons and therefore abolishes these reflexes. Immunocytochemical and pharmacological experiments thus suggest that inhibition by some spiking local interneurons may be mediated by GABA.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Society for Neuroscience
ISSN: 0270-6474
Date of First Compliant Deposit: 31 July 2018
Date of Acceptance: 2 October 1986
Last Modified: 02 May 2019 11:28
URI: http://orca-mwe.cf.ac.uk/id/eprint/113774

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