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The interactions of optoelectronically active molecules and complexes with (bio) macromolecules for applications in biosensors and directed assembly

Al-Azzawi, Ammar 2017. The interactions of optoelectronically active molecules and complexes with (bio) macromolecules for applications in biosensors and directed assembly. PhD Thesis, Cardiff University.
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Abstract

Since the discovery of DNA as the template for protein synthesis, great effort has been made by researchers to develop DNA as a target for drugs and biosensors. For biosensors small molecules DNA-binder are required that change chemical and physical properties (such as absorbance spectra, redox properties) on binding. Proteins and enzymes are still a predominant target for disease therapy. However, DNA can also be a target for medicine.1 In fact,small molecules which bind to DNA function as drugs or as sensitisers in biosensors. This chapter gives a general overview of the DNA structure. Chapter one starts by introducing some important concepts of development of biosensors. The chapter discusses DNA functions and the mode of interaction of small molecules with doublestranded DNA as well as the types of binding, such as electrostatic, intercalation and groove interactions. Moreover, the chapter briefly describes supramolecular and self-assembled nanostructured materials based on natural and synthetic polymers. The chapter finishes with the biophysical techniques which can be used to quantify the interaction between molecules and duplex DNA, such as UV-visible spectroscopy, circular dichroism (CD) and isothermaltitration calorimetry (ITC).

Item Type: Thesis (PhD)
Status: Unpublished
Schools: Chemistry
Date of First Compliant Deposit: 20 June 2018
Last Modified: 08 Aug 2019 02:05
URI: http://orca-mwe.cf.ac.uk/id/eprint/112595

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