Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Release of endothelium-derived relaxing factor is inhibited by 8-bromo-cyclic guanosine monophosphate

Evans, H. G., Warden-Smith, Jeremy and Lewis, Malcolm John 1988. Release of endothelium-derived relaxing factor is inhibited by 8-bromo-cyclic guanosine monophosphate. Journal of Cardiovascular Pharmacology 12 (6) , pp. 672-677.

Full text not available from this repository.

Abstract

Endothelial cells are known to contain both soluble and particulate guanylate cyclase, but the functional role of cyclic guanosine monophosphate (cGMP) in endothelial cells remains unknown. We have investigated the effects of 8-bromo-cGMP on endothelium-dependent relaxations to acetylcholine, substance P, ATP, and the calcium ionophore A23187, and on endothelium-independent relaxations to sodium nitroprusside and glyceryl trinitrate (GTN). The ability of each of these agents to relax phenylephrine-preconstricted rings of rabbit aorta was tested in the absence and presence of 8-bromo-cGMP. In the presence of 8-bromo-cGMP, a greater concentration of phenylephrine had to be used to produce a similar level of tone and then endothelium-dependent relaxations to acetylcholine and substance P were inhibited, whereas endothelium-dependent relaxations to ATP and A23187 were unaffected. Endothelium-independent relaxations to sodium nitroprusside and GTN were only inhibited at the highest concentrations of nitroprusside and GTN. These results suggest that: (a) increasing GMP levels in endothelial cells inhibit agonist-induced release of endothelium-derived relaxing factor (EDRF); (b) a negative feedback mechanism may exist whereby EDRF stimulates soluble guanylate cyclase in endothelial cells to inhibit its own release; and (c) ATP does not induce EDRF release via phosphoinositol hydrolysis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Medicine
Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
ISSN: 0160-2446
Last Modified: 04 Jun 2017 02:12
URI: http://orca-mwe.cf.ac.uk/id/eprint/10870

Citation Data

Cited 33 times in Google Scholar. View in Google Scholar

Cited 14 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item