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Diagnosis of aspergillosis by PCR: clinical considerations and technical tips

Barnes, Rosemary A., White, P. Lewis, Morton, C. Oliver, Rogers, Thomas R, Cruciani, Mario, Loeffler, Juergen and Donnelly, J. Peter 2018. Diagnosis of aspergillosis by PCR: clinical considerations and technical tips. Medical Mycology 56 (S1) , S60-S72. 10.1093/mmy/myx091

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Standardization of Aspergillus polymerase chain reaction (PCR) protocols has progressed, and analytical validity of blood-based assays has been formally established. It remains necessary to consider how the tests can be used in practice to maximize clinical utility. To determine the optimal diagnostic strategies and influence on patient management, several factors require consideration, including the patient population, incidence of invasive aspergillosis (and other fungal disease), and the local antifungal prescribing policy. Technical issues such as specimen type, ease of sampling, frequency of testing, access to testing centers, and time to reporting will also influence the use of PCR in clinical practice. Interpretation of all diagnostic tests is dependent on the clinical context and molecular assays are no exception, but with the proposal to incorporate Aspergillus PCR into the second revision of the consensus guidelines for defining invasive fungal disease the acceptance and understanding of molecular tests should improve.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Informa Healthcare / Wiley: No OnlineOpen / Oxford University Press (OUP)
ISSN: 1369-3786
Funders: none
Date of First Compliant Deposit: 27 November 2017
Date of Acceptance: 29 August 2017
Last Modified: 27 Oct 2018 07:07

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