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Decreased pigment epithelium-derived factor expression in human breast cancer progression

Cai, Jun, Parr, Christian, Watkins, Gareth, Jiang, Wen Guo and Boulton, Michael Edwin 2006. Decreased pigment epithelium-derived factor expression in human breast cancer progression. Clinical Cancer Research 12 (11) , pp. 3510-3517. 10.1158/1078-0432.CCR-06-0094

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Abstract

Purpose: The aim of this study was to correlate the expression of pigment epithelium–derived factor (PEDF), a potent endogenous antiangiogenic molecule, with severity and prognosis in breast cancer. Experimental Design: To investigate the gene expression profile of PEDF in human breast cancer in relation to a patient's clinical variables, we examined human breast cancer tissue (n = 119), background breast tissue (n = 33), and a range of cell lines for mRNA and protein levels of PEDF by using reverse transcription PCR, real-time quantitative PCR, immunohistochemistry, and ELISA. Results: By using reverse transcription PCR, real-time quantitative PCR, immunohistochemistry, and ELISA, PEDF expression was found to be dramatically decreased in breast cancer. An overall outlook for the patients inversely correlated with PEDF mRNA levels. Exogenous PEDF inhibits endothelial tubule formation induced by breast cancer cell–conditioned medium, in vitro. Conclusion: These observations collectively support the hypothesis that a lack of PEDF expression is a potent factor for the enhancement of tumor growth and angiogenesis in breast cancer.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Optometry and Vision Sciences
Publisher: American Association for Cancer Research
ISSN: 1078-0432
Last Modified: 18 Oct 2017 08:13
URI: http://orca-mwe.cf.ac.uk/id/eprint/107

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