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Hybrid synthetic receptors on MOSFET devices for detection of prostate specific antigen in human plasma

Tamboli, Vibha K., Bhalla, Nikhil, Jolly, Pawan, Bowen, Chris R., Taylor, John T., Bowen, Jenna, Allender, Christopher and Estrela, Pedro 2016. Hybrid synthetic receptors on MOSFET devices for detection of prostate specific antigen in human plasma. Analytical Chemistry 88 (23) , pp. 11486-11490. 10.1021/acs.analchem.6b02619

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Abstract

The study reports the use of extended gate field-effect transistors (FET) for the label-free and sensitive detection of prostate cancer (PCa) biomarkers in human plasma. The approach integrates for the first time hybrid synthetic receptors comprising of highly selective aptamer-lined pockets (apta-MIP) with FETs for sensitive detection of prostate specific antigen (PSA) at clinically relevant concentrations. The hybrid synthetic receptors were constructed by immobilizing an aptamer–PSA complex on gold and subjecting it to 13 cycles of dopamine electropolymerization. The polymerization resulted in the creation of highly selective polymeric cavities that retained the ability to recognize PSA post removal of the protein. The hybrid synthetic receptors were subsequently used in an extended gate FET setup for electrochemical detection of PSA. The sensor was reported to have a limit of detection of 0.1 pg/mL with a linear detection range from 0.1 pg/mL to 1 ng/mL PSA. Detection of 1–10 pg/mL PSA was also achieved in diluted human plasma. The present apta-MIP sensor developed in conjunction with FET devices demonstrates the potential for clinical application of synthetic hybrid receptors for the detection of clinically relevant biomarkers in complex samples.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Publisher: American Chemical Society
ISSN: 0003-2700
Date of First Compliant Deposit: 27 October 2017
Date of Acceptance: 18 November 2016
Last Modified: 12 Jan 2018 01:14
URI: http://orca-mwe.cf.ac.uk/id/eprint/106014

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