Pearson, James A. ORCID: https://orcid.org/0000-0002-2867-2269 and Wong, Florence Susan ORCID: https://orcid.org/0000-0002-2812-8845 2015. Identification of islet antigen-specific CD8 T cells using MHCI-peptide tetramer reagents in the non obese diabetic (NOD) mouse model of type 1 diabetes. Gillespie, Kathleen M., ed. Type-1 Diabetes, Vol. 1433. Methods in Molecular Biology, Springer, pp. 119-125. (10.1007/7651_2015_295) |
Official URL: http://dx.doi.org/10.1007/7651_2015_295
Abstract
MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.
Item Type: | Book Section |
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Date Type: | Published Online |
Status: | Published |
Schools: | Medicine |
Uncontrolled Keywords: | Antigen-specific; Flow cytometry; MHCI-peptide tetramer staining; T cell receptor. |
Publisher: | Springer |
ISBN: | 9781493936410 |
ISSN: | 1064-3745 |
Last Modified: | 02 Nov 2022 11:49 |
URI: | https://orca.cardiff.ac.uk/id/eprint/103212 |
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