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Marina Cavaiuolo, Giacomo Cocetta, Natasha Damiana Spadafora, Carsten T. Müller, Hilary J. Rogers, Antonio Ferrante
Gene expression analysis of rocket salad under pre-harvest and postharvest stresses: A transcriptomic resource for Diplotaxis tenuifolia
www.plosone.org
www.plosone.org
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0.8504 0 Td
(impor-)Tj
-33.7037 -1.2982 Td
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2.9197 0 Td
(the)Tj
1.4683 0 Td
(rapid)Tj
2.3358 0 Td
(deterioration)Tj
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(and)Tj
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(short)Tj
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(shelf-life)Tj
3.6453 0 Td
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1.0148 0 Td
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(well)Tj
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(their)Tj
2.1089 0 Td
(nutritional)Tj
4.5297 0 Td
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/F14 1 Tf
2.0239 0 Td
[(\n)17()20( )10( )10()4()7()6()7(&)7()6(&)]TJ
/F9 1 Tf
5.0626 0 Td
(species,)Tj
3.2202 0 Td
(known)Tj
3.0047 0 Td
(as)Tj
1.0148 0 Td
(rocket)Tj
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(\()Tj
/F14 1 Tf
[()16()-9(\r)4()7()]TJ
2.8517 0 Td
[( )-3()22(')7()15(\()3()]TJ
/F9 1 Tf
2.5228 0 Td
(and)Tj
/F14 1 Tf
1.7291 0 Td
[()-4()10()10(\))4()12(')11(*)-4( )]TJ
4.2066 0 Td
[(')11(&)2()18(\r)12()12(+)9()10(\))12()4()]TJ
/F9 1 Tf
(\))Tj
4.2916 0 Td
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1.0148 0 Td
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(commercial)Tj
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([)Tj
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(28)Tj
0 g
(].)Tj
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(effi-)Tj
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2.5398 0 Td
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4.7735 0 Td
(storage,)Tj
3.3109 0 Td
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2.1203 0 Td
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1.3833 0 Td
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(crops)Tj
2.3981 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
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-27.3484 -1.2983 Td
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3.6113 0 Td
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1.8029 0 Td
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[()-4()10()10(\))4()12(')11(*)-4( )]TJ
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[(')11(&)2()18(\r)12()12(+)9()10(\))12()4()]TJ
/F9 1 Tf
(\))Tj
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(for)Tj
1.389 0 Td
(24h)Tj
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2.1486 0 Td
(secondary)Tj
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(responses,)Tj
-32.5415 -1.2982 Td
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2.3981 0 Td
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(have)Tj
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3.7814 0 Td
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3.1351 0 Td
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3.9911 0 Td
(are)Tj
1.4513 0 Td
(still)Tj
1.6385 0 Td
(unimpaired)Tj
4.9209 0 Td
([)Tj
0.83 0.64 0.02 0 k
(30)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(31)Tj
0 g
(].)Tj
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 525.2031 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
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4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F0 1 Tf
-6.4961 -1.5023 Td
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2.4708 0 Td
(materials)Tj
4.2284 0 Td
(and)Tj
1.8662 0 Td
(stress)Tj
2.863 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 490.2236 cm
BT
/F14 1 Tf
1 TL
-0.004 Tc
0 0 Td
[()-4()10()10(\))4()12(')11(*)-4( )]TJ
4.2009 0 Td
[(')11(&)2()18(\r)12()12(+)9()10(\))12()4()]TJ
/F9 1 Tf
3.9571 0 Td
(cv)Tj
1.0998 0 Td
(Frastagliata)Tj
4.7906 0 Td
(plants)Tj
2.6248 0 Td
(were)Tj
2.1203 0 Td
(grown)Tj
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1.0374 0 Td
(controlled)Tj
4.32 0 Td
(growth)Tj
-33.1991 -1.3039 Td
(chambers)Tj
4.0875 0 Td
(\(26C;)Tj
2.7893 0 Td
(400)Tj
1.6497 0 Td
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ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 303.137 481.6629 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 309.3165 477.1842 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4422 0 Td
(16)Tj
1.1735 0 Td
(h)Tj
0.7483 0 Td
(photoperiod;)Tj
5.4142 0 Td
(relative)Tj
3.1407 0 Td
(humidity)Tj
3.9062 0 Td
(6070%\))Tj
3.7417 0 Td
(for)Tj
1.3833 0 Td
(30)Tj
1.1735 0 Td
(days)Tj
1.9956 0 Td
(post-)Tj
-34.0495 -1.2982 Td
(sowing.)Tj
3.2769 0 Td
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2.4321 0 Td
(were)Tj
2.1203 0 Td
(sown)Tj
2.3244 0 Td
(in)Tj
1.0317 0 Td
(polystyrene)Tj
4.8302 0 Td
(trays)Tj
2.1373 0 Td
(within)Tj
2.8119 0 Td
(tanks)Tj
2.3584 0 Td
(using)Tj
2.3925 0 Td
(perlite)Tj
2.7666 0 Td
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1.0148 0 Td
(growth)Tj
3.0727 0 Td
(substrate.)Tj
-32.5698 -1.2983 Td
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2.6702 0 Td
(were)Tj
2.1203 0 Td
(filled)Tj
2.2224 0 Td
(with)Tj
1.9956 0 Td
(nutrient)Tj
3.5035 0 Td
(solution)Tj
3.4809 0 Td
(composed)Tj
4.3086 0 Td
(of)Tj
1.0148 0 Td
(\(concentrations)Tj
6.5197 0 Td
(expressed)Tj
4.0932 0 Td
(in)Tj
1.0374 0 Td
(mM\):)Tj
2.4945 0 Td
(13)Tj
-35.4611 -1.3039 Td
(N-NO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 225.6944 436.5354 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 229.2661 438.1795 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4422 0 Td
(1.5)Tj
1.3889 0 Td
(P,)Tj
1.0035 0 Td
(8)Tj
0.686 0 Td
(K,)Tj
1.1168 0 Td
(3.5)Tj
1.3946 0 Td
(Ca,)Tj
1.5364 0 Td
(1.7)Tj
1.3946 0 Td
(Mg,)Tj
1.7972 0 Td
(9.5)Tj
1.389 0 Td
(Na,)Tj
1.6157 0 Td
(8.0)Tj
1.389 0 Td
(Cl,)Tj
1.3493 0 Td
(2.7)Tj
1.3946 0 Td
(S,)Tj
0.9184 0 Td
(0.04)Tj
1.8652 0 Td
(Fe)Tj
1.1679 0 Td
(and)Tj
1.7234 0 Td
(micronutrients)Tj
6.2532 0 Td
(\(Hoagland's)Tj
-32.7512 -1.2982 Td
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4.0364 0 Td
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3.2825 0 Td
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2.1147 0 Td
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3.1407 0 Td
(for)Tj
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(24h)Tj
1.6894 0 Td
(after)Tj
2.0239 0 Td
(30)Tj
1.1622 0 Td
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3.2882 0 Td
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-34.1742 -1.2983 Td
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1.1848 0 Td
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2.1883 0 Td
(nutrient)Tj
3.5036 0 Td
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1.0148 0 Td
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3.2825 0 Td
(\(1\))Tj
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(salinity:)Tj
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(con-)Tj
-35.0869 -1.3039 Td
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3.05 0 Td
(250)Tj
1.6441 0 Td
(mM)Tj
1.9332 0 Td
(NaCl;)Tj
2.5228 0 Td
(\(2\))Tj
1.3776 0 Td
(root-imposed)Tj
5.6749 0 Td
(heat)Tj
1.9049 0 Td
(stress:)Tj
2.6249 0 Td
(heating)Tj
3.1804 0 Td
(to)Tj
1.0262 0 Td
(40C)Tj
2.228 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(water)Tj
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(bath;)Tj
2.211 0 Td
(\(3\))Tj
1.3777 0 Td
(N-)Tj
-34.8772 -1.2983 Td
(starvation:)Tj
4.4163 0 Td
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2.931 0 Td
(of)Tj
1.0148 0 Td
(nitrogen)Tj
3.651 0 Td
(\(-NO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 341.8582 384.548 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(3)Tj
0.4762 0.9185 Td
(-)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(/-NH)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 369.8645 384.548 cm
BT
/F9 1 Tf
1 TL
-0.0053 Tc
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(4)Tj
0.4762 0.9185 Td
(+)Tj
ET
Q
q
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1 J
0 w
10 0 0 10 377.7448 386.1921 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
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(\).)Tj
0.788 0 Td
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4.8132 0 Td
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3.1804 0 Td
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(to)Tj
1.0261 0 Td
(freshly)Tj
2.8914 0 Td
(har-)Tj
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2.5625 0 Td
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1.0148 0 Td
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(\(1\))Tj
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(plastic)Tj
2.7666 0 Td
(boxes)Tj
2.4832 0 Td
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(the)Tj
1.4684 0 Td
(dark)Tj
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(at)Tj
-36.4362 -1.2983 Td
(4C;)Tj
1.9729 0 Td
(\(2\))Tj
1.3777 0 Td
(wounding:)Tj
4.524 0 Td
(leaves)Tj
2.5625 0 Td
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2.1203 0 Td
(cut)Tj
1.4684 0 Td
(into)Tj
1.8425 0 Td
(46)Tj
1.6894 0 Td
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1.7291 0 Td
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2.7099 0 Td
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(the)Tj
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2.7041 0 Td
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(open)Tj
2.2166 0 Td
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2.7666 0 Td
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(in)Tj
1.0375 0 Td
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(dark)Tj
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0.9524 0 Td
(20C;)Tj
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(\(4\))Tj
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2.2734 0 Td
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2.6362 0 Td
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1.0375 0 Td
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2.4831 0 Td
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1.0375 0 Td
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1.4682 0 Td
(dark)Tj
2.0523 0 Td
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0.9524 0 Td
(20C.)Tj
2.4492 0 Td
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3.3618 0 Td
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1.389 0 Td
(pre-)Tj
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(and)Tj
1.7291 0 Td
(post-)Tj
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3.0898 0 Td
(stress)Tj
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4.4957 0 Td
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2.1203 0 Td
(collected)Tj
3.7417 0 Td
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2.2052 0 Td
(untreated)Tj
4.0706 0 Td
(plants)Tj
2.6248 0 Td
(to)Tj
1.0262 0 Td
(account)Tj
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(for)Tj
1.3833 0 Td
(any)Tj
1.6611 0 Td
(circadian)Tj
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(rhythms)Tj
3.5773 0 Td
(and)Tj
1.7348 0 Td
(ensure)Tj
2.8743 0 Td
(that)Tj
1.7745 0 Td
(they)Tj
1.9276 0 Td
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2.1203 0 Td
(completely)Tj
4.5806 0 Td
(unstressed.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 281.9905 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(RNA-Seq)Tj
4.3984 0 Td
(and)Tj
1.8662 0 Td
(bioinformatic)Tj
5.8158 0 Td
(analysis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 264.9826 cm
BT
/F9 1 Tf
1 TL
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2.313 0 Td
(RNA)Tj
2.262 0 Td
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1.7008 0 Td
(extracted)Tj
3.8835 0 Td
(from)Tj
2.211 0 Td
(1)Tj
0.6916 0 Td
(g)Tj
0.686 0 Td
(of)Tj
1.0148 0 Td
(leaf)Tj
1.6101 0 Td
(powder)Tj
3.2541 0 Td
(using)Tj
2.3868 0 Td
(the)Tj
1.474 0 Td
(hot)Tj
1.5477 0 Td
(borate)Tj
2.7609 0 Td
(method)Tj
3.3279 0 Td
([)Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
(].)Tj
2.0749 0 Td
(RNA)Tj
-33.1991 -1.2982 Td
(purity)Tj
2.6532 0 Td
(and)Tj
1.7348 0 Td
(integrity)Tj
3.6113 0 Td
(were)Tj
2.1259 0 Td
(assessed)Tj
3.4696 0 Td
(with)Tj
1.9956 0 Td
(an)Tj
1.2019 0 Td
(Agilent)Tj
3.1578 0 Td
(2100)Tj
2.1203 0 Td
(bioanalyzer-RNA)Tj
7.2169 0 Td
(6000)Tj
2.126 0 Td
(Nano)Tj
2.4434 0 Td
(Chip)Tj
-33.8567 -1.2983 Td
(\(Agilent)Tj
3.4922 0 Td
(Technologies\))Tj
5.8734 0 Td
(and)Tj
1.7291 0 Td
(Nanodrop)Tj
4.3653 0 Td
(8000)Tj
2.126 0 Td
(\(Thermo)Tj
3.8211 0 Td
(Scientific\).)Tj
4.4673 0 Td
(RNA)Tj
2.2621 0 Td
(with)Tj
1.9955 0 Td
(A260/A280)Tj
/F15 1 Tf
0 Tc
4.7736 0 Td
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1.3436 0 Td
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2.1146 0 Td
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