IL-7Rα expression regulates murine dendritic cell sensitivity to thymic stromal lymphopoietin

Kummola, Laura, Ortutay, Zsuzsanna, Chen, Xi, Caucheteux, Stephane, Hämäläinen, Sanna, Aittomäki, Saara, Yagi, Ryoji, Zhu, Jinfang, Pesu, Marko, Paul, William E. and Junttila, Ilkka S. 2017. IL-7Rα expression regulates murine dendritic cell sensitivity to thymic stromal lymphopoietin. The Journal of Immunology 198 (10) , pp. 3909-3918. 10.4049/jimmunol.1600753

Full text not available from this repository.

Abstract

Thymic stromal lymphopoietin (TSLP) and IL-7 are related cytokines that mediate growth and differentiation events in the immune system. They signal through IL-7Rα–containing receptors. Target cells of TSLP in Th2 responses include CD4 T cells and dendritic cells (DCs). Although it has been reported that expression of TSLP receptor (TSLPR) on CD4 T cells is required for OVA-induced lung inflammation, DCs have also been shown to be target cells of TSLP. In this study, we show that murine ex vivo splenic DCs are unresponsive to TSLP, as they fail to phosphorylate STAT5, but in vitro overnight culture, especially in presence of IL-4, renders DCs responsive to both TSLP and IL-7. This induced responsiveness is accompanied by dramatic upregulation of IL-7Rα on DCs with little change in expression of TSLPR or of γc. In splenic DCs, the induction of IL-7Rα occurs mainly in CD8− DCs. In vivo, we found that IL-4 has a differential regulatory role on expression of IL-7Rα depending on the cell type; IL-4 decreases IL-7Rα expression on CD4 T cells whereas it upregulates the expression on DCs. Our results indicate that the induction of IL-7Rα expression on DCs is critical for TSLP responsiveness and that IL-4 can upregulate IL-7Rα on DCs.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	American Association of Immunologists
ISSN:	0022-1767
Funders:	NIAID, University of Tampere
Date of Acceptance:	13 March 2017
Last Modified:	08 Jan 2020 03:27
URI:	https://orca.cardiff.ac.uk/id/eprint/101802

Citation Data

Cited 11 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item