α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction

Jurado, Jaime, De Navascues Melero, Joaquin ORCID: https://orcid.org/0000-0002-5414-4056 and Gorfinkiel, Nicole 2016. α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction. Journal of Cell Science , jcs.193268. 10.1242/jcs.193268

Preview

PDF - Accepted Post-Print Version Download (4MB) | Preview

Abstract

We have investigated how cell contractility and adhesion are functionally integrated during epithelial morphogenesis. To this end, we have analysed the role of α-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. We find that α-Catenin regulates pulsatile apical contraction in the amnioserosa, the main force-generating tissue driving closure of the embryonic epidermis. α-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. Furthermore, we uncover a genetic interaction between α-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module operating in this tissue.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences European Cancer Stem Cell Research Institute (ECSCRI)
Publisher:	The Company of Biologists Ltd
ISSN:	0021-9533
Date of First Compliant Deposit:	26 June 2017
Date of Acceptance:	2 November 2016
Last Modified:	05 May 2023 03:45
URI:	https://orca.cardiff.ac.uk/id/eprint/101732

Citation Data

Cited 30 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item