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Isolation of nucleic acids for fungal diagnosis

White, P. Lewis and Barnes, Rosemary Ann 2017. Isolation of nucleic acids for fungal diagnosis. In: Lion, Thomas ed. Human Fungal Pathogen Identification: Methods and Protocols, Vol. 1508. Methods in Molecular Biology, Springer, pp. 223-247. (10.1007/978-1-4939-6515-1_12)

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Abstract

PCR can aid in the diagnosis of invasive fungal disease (IFD). While the large number of “in-house” methodologies drives technological diversity, providing robustness, they make it difficult to identify optimal strategies, limiting standardization, and widespread acceptance. No matter how efficient, PCR utility will be limited by the quality of extracted nucleic acid. This chapter highlights benefits and limitations affecting the nucleic acid extraction process, before focusing on recent recommendations that through multicenter evaluation have provided optimal and standardized methodology.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Springer
ISBN: 9781493965137
ISSN: 10643745
Date of First Compliant Deposit: 31 May 2017
Date of Acceptance: 30 June 2016
Last Modified: 05 Jun 2017 15:14
URI: http://orca-mwe.cf.ac.uk/id/eprint/101013

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