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OPN-a splicing variant expression in non-small cell lung cancer and its effects on the bone metastatic abilities of lung cancer cells in vitro

Hao, Chengcheng, Cui, Yuxin, Hu, M., Zhi, X., Zhang, Lijian, Li, Wenbin, Wu, Wei, Cheng, Shan and Jiang, Wen Guo 2017. OPN-a splicing variant expression in non-small cell lung cancer and its effects on the bone metastatic abilities of lung cancer cells in vitro. Anticancer Research 37 (5) , pp. 2245-2254. 10.21873/anticanres.11561

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Abstract

Background: Osteopontin (OPN) is known to be involved in the development of certain cancers, including non-small cell lung cancer (NSCLC). However, its role in tumour progression remains unclear. The present study investigated the expression and biological impact of the OPN variant, OPN-a in NSCLC. Materials and Methods: OPN-a splicing variant expression in human NSCLC tissues was analyzed by real-time qPCR and immunohistochemistry (IHC), respectively. The impact of OPN-a on cellular functions of lung cancer cells was also evaluated. In addition, an in vitro model was developed for the assessment of interactions between lung cancer cells and bone tissue. Results: The expression of OPN-a was higher in lung cancer tissues compared to normal controls. OPN-a promoted the malignant phenotypes of A549 cells by enhancing cell-adherent abilities to bone tissues, which could be mediated by the interaction with the cell surface receptor αvβ3 integrin. Conclusion: OPN-a may represent a bone metastatic factor in human lung cancer, as well as a potential therapy target.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: International Institute of Anticancer Research
ISSN: 0250-7005
Funders: Cancer Research Wales, Cardiff China Medical Scholarship
Date of First Compliant Deposit: 29 January 2018
Date of Acceptance: 5 April 2017
Last Modified: 27 Mar 2019 22:21
URI: http://orca-mwe.cf.ac.uk/id/eprint/100480

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